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However, these mainstream therapies face critical limitations including the rapid emergence of drug resistance, FKS1/FKS2 gene mutations reducing β-1,3-glucan synthase affinity, Targeted enzyme (Erg11p) modification, overexpression of efflux pumps (CDR1, MDR1), and upregulation of the ergosterol pathway mediate azole resistance (Pristov and Ghannoum, 2019)
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