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While the functional unit is a homotrimer in membranes, the dominant catalytically active organization of soluble GSTs is a dimer
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Quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR) Total RNA was isolated from HepG2 cells or liver tissues utilizing RNAiso plus (Takara, Kyoto, Japan), after which cDNA synthesis was conducted using a PrimeScript-RT-reagent-kit (Takara, Kyoto, Japan)
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