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Instead, strategies that simultaneously address the multiple root causes of NAD+ decline such as the combined administration of a NAD+ precursor, a CD38 inhibitor, and an NAMPT activator, hold potential for NAD+ restoration with greater measurable benefits (Fig

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5 μL CCK8 solution (Beyotime, C0038, China) was added to each well, and after 3 h of incubation, the absorbance at 450 nm was measured using a microplate reader (Thermo, USA)