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Accordingly, ESCRT-I, ESCRT-III and ALIX proteins were shown to rapidly localized to the lysosome membrane following treatment with LLOME or GPN [44, 45]

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We used a semiquantitative neuropathological scoring system, and the sum of the analyzed affected areas (0–4) (i) and karyopyknotic cells in the brain areas (0–4) (ii) made (i) + (ii) a combined score (0–8) as follows