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These activated microglia release cytokines, chemokines, and cytotoxic substances that induce cellular responses, ultimately altering central excitability and neuroplasticity [39, 40]

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Xiaoyaosan decreases the expression of purinergic ligand-gated ion channel 7 (P2X7) protein, inhibits the activation of the NLRP3 inflammasome, and reduces the levels of proinflammatory cytokines such as TNF-α, IL-1β, and IL-6 ( Bacteroides in the gut of CUMS rats, inhibited the excessive activation of the TLR4/NLRP3 signaling pathway in the colon and brain, downregulated NF-κB expression, and improved gastrointestinal symptoms and depressive behaviors ( Chaihu-Shugan-San is another TCM formula used to soothe the liver and relieve depression